法国euromedex细菌双杂交系统试剂盒EUK001-中国授权总代理:法国...

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The BACTH system has been developed by the group of Dr. D. Ladant at the Pasteur Institute and is based on the interaction-mediated reconstitution of the adenylate cyclase activity in E. coli (1). It exploits the fact that the catalytic domain of adenylate cyclase (CyaA) from Bordetella pertussis (2) consists of two complementary fragments, T25 and T18 (Figure 1A), that are not active when physically separated (Figure 1B). When these two fragments are fused to interacting polypeptides, X and Y, heterodimerization of these hybrid proteins results in functional complementation between T25 and T18 fragments and, therefore, cAMP synthesis (Figure 1C). Cyclic AMP produced by the reconstituted chimeric enzyme binds to the catabolite activator protein, CAP. The cAMP/CAP complex is a pleiotropic regulator of gene transcription in E. coli. It turns on the expression of several resident genes, including genes of the lac and mal operons involved in lactose and maltose catabolism (Figure 1D). Therefore, bacteria become able to utilize lactose or maltose as the unique carbon source and can be easily distinguished on indicator or selective media.
品牌:EUROMEDEX 产地:法国 规格:kit 关键字:Bacterial Adenylate Cyclase-based Two-Hybrid 该厂家的产品,我这边都是独家代理,你可以来电咨询, ...法国euromedex细菌双杂交系统试剂盒EUK001法国euromedex细菌双杂交系统试剂盒EUK001细菌双杂交系统试剂盒（腺苷酸环化酶）EUK001细菌双杂交系统试剂盒（腺苷酸环化酶）( 英文名称：BACTH System Kit)货号：EUK001品牌：EUROMEDEX产地：法国规格：kit关键字：...法国euromedex细菌双杂交系统试剂盒EUK001